The effect of actinomycin D in vivo upon peripheral nucleoli and other nuclear organelles in oocytes of Triturus cristatus.
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چکیده
منابع مشابه
Tritiated Actinomycin D as a Cytochemical Label for Small Amounts of Dna
Actinomycin D blocks DNA-dependent RNA synthesis by binding to DNA (Reich et al., reference 15). On a cellular level, several radioautographic studies have demonstrated the in vivo binding sites of radioactive actinomycin (de Vitry, reference 19; Fraccaro et al., reference 3; Goldstein et al., reference 8; Ro et al., reference 16; Rothstein et al., reference 17; Sonneborn and Rothstein, referen...
متن کاملThe role of lampbrush chromosomes in the formation of nucleoli in amphibian oocytes.
Theories concerning the mode of origin of peripheral nucleoli in amphibian oocytes have been examined and tested. In Triturus cristatus the giant fusing loops of the 3 shortest lampbrush bivalents resemble nucleoli when viewed in phase contrast and may be considered as possible sites of production of nucleoli. Giant fusing loops, however, differ from peripheral nucleoli in certain important res...
متن کاملEffects of actinomycin D on the association of newly formed ribonucleoproteins with the cistrons of ribosomal RNA in Triturus oocytes
The effect of actinomycin D(AMD) on the association of the nascent ribonucleo-protein (RNP) fibrils containing the precursors of ribosomal RNA (pre-rRNA) with their template deoxyribonucleoprotein (rDNP) strands has been studied in lampbrush stage oocytes from Triturus alpestris. Ovary pieces were incubated in vitro either in media containing radioactive ribonucleosides and then, for various ti...
متن کاملHigh resolution scanning electron microscopy of the nuclear envelope: demonstration of a new, regular, fibrous lattice attached to the baskets of the nucleoplasmic face of the nuclear pores
The nuclear envelope (NE) of amphibian oocytes can be readily isolated in relatively structurally intact and pure form and has been used extensively for structural studies. Using high resolution scanning electron microscopy (HRSEM), both surfaces of the NE can be visualized in detail. Here, we demonstrate the use of HRSEM to obtain high resolution information of NE structure, confirming previou...
متن کاملSperm DNA damage in mice irradiated with various doses of X-rays alone or in combination with actinomycin D or bleomycin sulfate: an in vivo study
Background: DNA damage in male germ cells due to exposure to environmental and manmade physico-chemical genotoxic agents is considered as the main cause of male infertility. The aim of this study was to evaluate the effects of combined modalities (radiotherapy and chemotherapy) routinely used for cancer treatment on mouse sperm chromatin in vivo. Materials and Methods: Forty-eight mice were div...
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ورودعنوان ژورنال:
- Journal of cell science
دوره 10 3 شماره
صفحات -
تاریخ انتشار 1972